Journal: Journal of Nucleic Acids

Article Title: Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNA/LNA Microarray

doi: 10.1155/2012/283560

Figure Lengend Snippet: Self-self hybridization at 56°C of 5 μ g (per dye) of labeled total mouse brain RNA. Diagrams of average intensity values show filtered results in logarithmic scale. The y axis represents values of AlexaFluor3 dye measurement at 532 nm, and the x axis represents values of AlexaFluor5 dye measurement at 635 nm. Red spots represent all signals from LNA and DNA probes spotted on the microarray slide. (a, c) Detection of MiR-9 (yellow spots, black arrow) and miR-9* (yellow spots, white arrow) with LNA probes. (b, d) Detection of snoRNAs (yellow spots) with antisense DNA probes (including mismatch probes in (d)). Sense DNA probes signals were below detection levels and thus filtered out. (a, b) RNA labeling using the protocol described by manufacturer. (c, d) Modified RNA labeling (see ).

Article Snippet: The DNA probes were purchased from Microsynth (Switzerland).

Techniques: Hybridization, Labeling, Microarray, Modification

Journal: Journal of Nucleic Acids

Article Title: Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNA/LNA Microarray

doi: 10.1155/2012/283560

Figure Lengend Snippet: Self-self hybridization at 56°C of 1 μ g (per dye) of labeled total mouse brain RNA. Diagrams of average intensity values show filtered results in logarithmic scale. The y axis represents values of AlexaFluor 3 dye measurement at 532 nm, and the x axis represents values of AlexaFluor5 dye measurement at 635 nm. Red spots represent all signals from LNA and DNA probes spotted on the microarray slide. Detection of (a) tRNAs (yellow spots) and (b) 7SK RNA (yellow spots) with antisense DNA probes (including mismatch and deletion probes). Sense DNA probes were below detection levels and thus filtered out. (c) Diagram showing mean intensity values ( y axis) of DNA probes for detection of highly structured ncRNAs.

Article Snippet: The DNA probes were purchased from Microsynth (Switzerland).

Techniques: Hybridization, Labeling, Microarray

Journal: Journal of Nucleic Acids

Article Title: Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNA/LNA Microarray

doi: 10.1155/2012/283560

Figure Lengend Snippet: (a) Mean intensity values of all antisense DNA probes detecting snoRNAs and 7SK RNA and LNA probes detecting mir-9 and miR-124 when 1 μ g (grey bars) or 2 μ g (black bars) of labeled total mouse brain RNA is used with a hybridization temperature of 64°C. (b) Percentage of mean intensity values for all antisense DNA probes detecting snoRNAs and 7SK RNA and LNA probes detecting mir-9. White bars: hybridization at 56°C with 1 μ g of labeled total mouse brain RNA; black bars: hybridization at 64°C with 2 μ g of labeled total mouse brain RNA.

Article Snippet: The DNA probes were purchased from Microsynth (Switzerland).

Techniques: Labeling, Hybridization

Journal: Journal of Nucleic Acids

Article Title: Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNA/LNA Microarray

doi: 10.1155/2012/283560

Figure Lengend Snippet: Self-self hybridization at 64°C of 2 μ g (per dye) of labeled total mouse brain RNA. Diagrams of average intensity values show filtered results in logarithmic scale. The y axis represents values of AlexaFluor3 dye measurement at 532 nm, and the x axis represents values of AlexaFluor5 dye measurement at 635 nm. Red spots represent all signals from LNA and DNA probes spotted on the microarray slide. (a) Detection of snoRNAs (yellow spots) with antisense DNA probes. (b) Detection of miR-9 (yellow spots, black arrows) and miR-9* (yellow spots, white arrow). Sense DNA probes were below detection levels and filtered out. (c) Diagram showing mean intensity values ( y axis) of all antisense DNA probes detecting snoRNAs.

Article Snippet: The DNA probes were purchased from Microsynth (Switzerland).

Techniques: Hybridization, Labeling, Microarray

Journal: Journal of Nucleic Acids

Article Title: Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNA/LNA Microarray

doi: 10.1155/2012/283560

Figure Lengend Snippet: Self-self hybridization at 64°C of 2 μ g (per dye) of labeled total mouse brain RNA. Diagrams of average intensity values show filtered results in logarithmic scale. The y axis represents values of AlexaFluor3 dye measurement at 532 nm, and the x axis represents values of AlexaFluor5 dye measurement at 635 nm. Red spots represent all signals from LNA and DNA probes spotted on the microarray slide. (a) Detection of snoZ39 (yellow spots) with antisense DNA probes (black arrow, blow up) and one nucleotide mismatch DNA probe (white arrow, blow up). (b) Diagram showing on the y axis the percentage of mean detection value of the antisense probe SNOZ39−6–60MM1 and the one nucleotide mismatch probe SNOZ39−6–60MM1. (c) Diagram showing the mean intensity values ( y axis) of the antisense probes of one (MM1) and two (MM2) nucleotide mismatch probes for 7SK RNA and SNORD55.

Article Snippet: The DNA probes were purchased from Microsynth (Switzerland).

Techniques: Hybridization, Labeling, Microarray